Hello I am willing to pay $80 for this assignment. It is a community college online programming 1 final. There are two instructional video demos: the links are https://www.3cmediasolutions.org/privid/122015?key=be5bcb131be7e7f04c59117269ef90a94692d014
and
https://www.3cmediasolutions.org/privid/127263?key=d9b5f78e5cc3f12b2364319dca32a71241e21d50
More instructions are within the zip file. They are in the index.html file as well as scattered throughout the actual java file.
It needs to be made on Eclipse IDE and named something like Assignment_5. My name is Aaron Choi, so please list that as the @author. He also requires a breif description/JavaDoc of the work done, which we have to put at the top, before the name. JavaDoc comments are indicated by /** before the comments. I have attached a screenshot with more instructions as well as the zip file containing all the files the professor provided. This is a beginner’s course, so please follow the intermediary/potentially unnecessary instructions he specifies.
Lastly, please make sure that after naming the file, you do not click finish and instead go to next, then under libraries and under classpath add an external jar. That jar will be the attached becker.zip file, which should be a jar once unzipped. Then, please do NOT make a modules path if prompted so after clicking finish during the creation of the new java project.
Please tell me if you need anything else.
Category: Undergraduate
Do references in APA form and also include the calculations in that and 2. Submit the results of your design in a neat and complete report, including a narrative description of the system, the sketches, a list of materials, and the analysis to show that your design meets the specifications.
For this assignment you will USE ONLY AWK to create a program for summarizing and printing information based on the directory listing data of files and information.
You are not to use any other programs, utilities, or scripting languages not covered in class, unless otherwise specifically and explicitly stated in this document.
Your submission should consist of exactly two files:
assign3.bash – a bash script used as the driver program for your awk script assign3.awk – the awk program used in assign3.bash
Note: again, do not use sed , Python, or any other languages or utilities not explicitly allowed by
this assignment.
For this assignment you will USE ONLY AWK to create a program for summarizing and printing information based on the directory listing data of files and information.
You are not to use any other programs, utilities, or scripting languages not covered in class, unless otherwise specifically and explicitly stated in this document.
Note: again, do not use sed , Python, or any other languages or utilities not explicitly allowed by
this assignment.our submission should consist of exactly two files:
assign3.bash – a bash script used as the driver program for your awk script
assign3.awk – the awk program used in assign3.bash
make sure 100%
1. One lesson learned by Whyte during his field work in Cornerville was that
(a) It is as important to be a tourist as it is to be a rigorous field researcher
(b) It is not necessary to be a rigorous field researcher because one can be a tourist
(c) It is important not to adopt the perspective of a tourist while doing field work
(d) It is important to study all available literature on slums before starting field work.
2. Whytes Saturday night participation in the bowling alley helped him see
(a) The impact of group structure on individual performance
(b) The relationship between bowling skills and gambling skills
(c) How bowling was far superior to both gambling and racketeering
(d) The impact of individual performance on group structure.
3. Whyte suggests that gender relations in Cornerville were
(a) As interesting and pleasant for the girls as it was for the men
(b) More interesting and pleasant for the girls than it was for the men
(c) Neither more nor less interesting and pleasant for the girls and for the men
(d) Less interesting and pleasant for the girls than it was for the men.
4. Whytes experience as a repeater
(a) Helped Whyte perform his field research carefully and repeatedly
(b) Helped Whyte understand the limits of immersion in the field
(c) Did not help Whyte repeat his field research in Cornerville
(d) Did not help Whyte understand how to relate with himself.
5. The Chief is without power in the Amerindian Societies discussed by Clastres
(a) Because Power has been internalized by these societies
(b) Because Power has been externalized by these societies
(c) Because Power has been identified with Culture in these societies
(d) Because Power is never meant to be exercised in these societies.
6. Clastres argues that Archaic Societies are societies of the mark because
(a) Everyone marked by torture bears the laws of Society on their bodies
(b) Everyone is tortured if they desire to leave society
(c) Everyone is marked by torture as a sign of the power of Nature
(d) Anyone marked by torture becomes superior in endurance to everyone else.
7. In Skolniks film the viewers may see clearly that
(a) Issues of Poverty and Inequality are never raised by anyone in Swaziland
(b) Issues of Poverty and Inequality are depoliticized in Swaziland
(c) Issues of Poverty and Inequality do not exist in Swaziland
(d) Issues of Poverty and Inequality are strongly politicized in Swaziland.
8. Lesotho and Swaziland have this in common. Both were
(a) Absolute Monarchies after they became independent States
(b) Poor provinces within Apartheid South Africa
(c) Former British Protectorates which became independent in the 1960s
(d) Places where culture had no importance in society.
9. Ferguson argues that
(a) All nation-states are the same in terms of political power
(b) All nation-states are pseudo-states
(c) All nation-states are either democracies or monarchies
(d) All nation-states are not the same in terms of political power.
10. Ferguson starts his essay with a joke told in Tijuana in order that those who read it
(a) Get a better understanding of the historical relations that bind US and Mexico
(b) Laugh when they hear it; and laugh again on being unable to understand it
(c) Get to know Tijuanas location in relation to the US border
(d) Get a better understanding of why building a wall with Mexico lies in US interests.
11. Ferguson points out the following:
(a) Lesotho is a real nation-state; but it does not have a currency of its own
(b) Lesotho is a real nation-state with its own currency called the maloti
(c) Lesotho is not a real nation-state in the political sense
(d) Lesotho and Transkei are both real nation-states.
12. Ferguson notes this about the Apartheid State in South Africa:
(a) Apartheid was hugely popular only when it was first introduced
(b) Apartheid was strongly contested by the White settlers in South Africa
(c) Apartheid was strongly contested by Black Africans in South Africa
(d) Apartheid came to an end at the same time as Bantustans were created.
13. Ferguson notes that Transkei was like Lesotho in that
(a) Both were part of South Africas migrant labor system
(b) Both were Bantustans
(c) Both were created by an Apartheid-State
(d) Both were economically independent of South Africa.
14. Ferguson argues that in Transkei
(a) Poverty was depoliticized, unlike in Lesotho
(b) Poverty was politicized, unlike in Lesotho
(c) A real nation-state was constructed, unlike in Lesotho
(d) Apartheid was abolished, unlike in Lesotho.
15. Ferguson is critical about this claim made by anthropologists:
(a) All cultures are the same; hence it is hard to choose a field to study
(b) All cultures are unique; hence it is easy to compare cultures
(c) All cultures are unique; hence it is hard to compare different cultures
(d) All cultures are either the same or different; hence it is hard to compare cultures.
please make sure 100% right thank you
1. One lesson learned by Whyte during his field work in Cornerville was that
(a) It is as important to be a tourist as it is to be a rigorous field researcher
(b) It is not necessary to be a rigorous field researcher because one can be a tourist
(c) It is important not to adopt the perspective of a tourist while doing field work
(d) It is important to study all available literature on slums before starting field work.
2. Whytes Saturday night participation in the bowling alley helped him see
(a) The impact of group structure on individual performance
(b) The relationship between bowling skills and gambling skills
(c) How bowling was far superior to both gambling and racketeering
(d) The impact of individual performance on group structure.
3. Whyte suggests that gender relations in Cornerville were
(a) As interesting and pleasant for the girls as it was for the men
(b) More interesting and pleasant for the girls than it was for the men
(c) Neither more nor less interesting and pleasant for the girls and for the men
(d) Less interesting and pleasant for the girls than it was for the men.
4. Whytes experience as a repeater
(a) Helped Whyte perform his field research carefully and repeatedly
(b) Helped Whyte understand the limits of immersion in the field
(c) Did not help Whyte repeat his field research in Cornerville
(d) Did not help Whyte understand how to relate with himself.
5. The Chief is without power in the Amerindian Societies discussed by Clastres
(a) Because Power has been internalized by these societies
(b) Because Power has been externalized by these societies
(c) Because Power has been identified with Culture in these societies
(d) Because Power is never meant to be exercised in these societies.
6. Clastres argues that Archaic Societies are societies of the mark because
(a) Everyone marked by torture bears the laws of Society on their bodies
(b) Everyone is tortured if they desire to leave society
(c) Everyone is marked by torture as a sign of the power of Nature
(d) Anyone marked by torture becomes superior in endurance to everyone else.
7. In Skolniks film the viewers may see clearly that
(a) Issues of Poverty and Inequality are never raised by anyone in Swaziland
(b) Issues of Poverty and Inequality are depoliticized in Swaziland
(c) Issues of Poverty and Inequality do not exist in Swaziland
(d) Issues of Poverty and Inequality are strongly politicized in Swaziland.
8. Lesotho and Swaziland have this in common. Both were
(a) Absolute Monarchies after they became independent States
(b) Poor provinces within Apartheid South Africa
(c) Former British Protectorates which became independent in the 1960s
(d) Places where culture had no importance in society.
9. Ferguson argues that
(a) All nation-states are the same in terms of political power
(b) All nation-states are pseudo-states
(c) All nation-states are either democracies or monarchies
(d) All nation-states are not the same in terms of political power.
10. Ferguson starts his essay with a joke told in Tijuana in order that those who read it
(a) Get a better understanding of the historical relations that bind US and Mexico
(b) Laugh when they hear it; and laugh again on being unable to understand it
(c) Get to know Tijuanas location in relation to the US border
(d) Get a better understanding of why building a wall with Mexico lies in US interests.
11. Ferguson points out the following:
(a) Lesotho is a real nation-state; but it does not have a currency of its own
(b) Lesotho is a real nation-state with its own currency called the maloti
(c) Lesotho is not a real nation-state in the political sense
(d) Lesotho and Transkei are both real nation-states.
12. Ferguson notes this about the Apartheid State in South Africa:
(a) Apartheid was hugely popular only when it was first introduced
(b) Apartheid was strongly contested by the White settlers in South Africa
(c) Apartheid was strongly contested by Black Africans in South Africa
(d) Apartheid came to an end at the same time as Bantustans were created.
13. Ferguson notes that Transkei was like Lesotho in that
(a) Both were part of South Africas migrant labor system
(b) Both were Bantustans
(c) Both were created by an Apartheid-State
(d) Both were economically independent of South Africa.
14. Ferguson argues that in Transkei
(a) Poverty was depoliticized, unlike in Lesotho
(b) Poverty was politicized, unlike in Lesotho
(c) A real nation-state was constructed, unlike in Lesotho
(d) Apartheid was abolished, unlike in Lesotho.
15. Ferguson is critical about this claim made by anthropologists:
(a) All cultures are the same; hence it is hard to choose a field to study
(b) All cultures are unique; hence it is easy to compare cultures
(c) All cultures are unique; hence it is hard to compare different cultures
(d) All cultures are either the same or different; hence it is hard to compare cultures.
Needs to be 100% correct very important thank you.
1. One lesson learned by Whyte during his field work in Cornerville was that
(a) It is as important to be a tourist as it is to be a rigorous field researcher
(b) It is not necessary to be a rigorous field researcher because one can be a tourist
(c) It is important not to adopt the perspective of a tourist while doing field work
(d) It is important to study all available literature on slums before starting field work.
2. Whytes Saturday night participation in the bowling alley helped him see
(a) The impact of group structure on individual performance
(b) The relationship between bowling skills and gambling skills
(c) How bowling was far superior to both gambling and racketeering
(d) The impact of individual performance on group structure.
3. Whyte suggests that gender relations in Cornerville were
(a) As interesting and pleasant for the girls as it was for the men
(b) More interesting and pleasant for the girls than it was for the men
(c) Neither more nor less interesting and pleasant for the girls and for the men
(d) Less interesting and pleasant for the girls than it was for the men.
4. Whytes experience as a repeater
(a) Helped Whyte perform his field research carefully and repeatedly
(b) Helped Whyte understand the limits of immersion in the field
(c) Did not help Whyte repeat his field research in Cornerville
(d) Did not help Whyte understand how to relate with himself.
5. The Chief is without power in the Amerindian Societies discussed by Clastres
(a) Because Power has been internalized by these societies
(b) Because Power has been externalized by these societies
(c) Because Power has been identified with Culture in these societies
(d) Because Power is never meant to be exercised in these societies.
6. Clastres argues that Archaic Societies are societies of the mark because
(a) Everyone marked by torture bears the laws of Society on their bodies
(b) Everyone is tortured if they desire to leave society
(c) Everyone is marked by torture as a sign of the power of Nature
(d) Anyone marked by torture becomes superior in endurance to everyone else.
7. In Skolniks film the viewers may see clearly that
(a) Issues of Poverty and Inequality are never raised by anyone in Swaziland
(b) Issues of Poverty and Inequality are depoliticized in Swaziland
(c) Issues of Poverty and Inequality do not exist in Swaziland
(d) Issues of Poverty and Inequality are strongly politicized in Swaziland.
8. Lesotho and Swaziland have this in common. Both were
(a) Absolute Monarchies after they became independent States
(b) Poor provinces within Apartheid South Africa
(c) Former British Protectorates which became independent in the 1960s
(d) Places where culture had no importance in society.
9. Ferguson argues that
(a) All nation-states are the same in terms of political power
(b) All nation-states are pseudo-states
(c) All nation-states are either democracies or monarchies
(d) All nation-states are not the same in terms of political power.
10. Ferguson starts his essay with a joke told in Tijuana in order that those who read it
(a) Get a better understanding of the historical relations that bind US and Mexico
(b) Laugh when they hear it; and laugh again on being unable to understand it
(c) Get to know Tijuanas location in relation to the US border
(d) Get a better understanding of why building a wall with Mexico lies in US interests.
11. Ferguson points out the following:
(a) Lesotho is a real nation-state; but it does not have a currency of its own
(b) Lesotho is a real nation-state with its own currency called the maloti
(c) Lesotho is not a real nation-state in the political sense
(d) Lesotho and Transkei are both real nation-states.
12. Ferguson notes this about the Apartheid State in South Africa:
(a) Apartheid was hugely popular only when it was first introduced
(b) Apartheid was strongly contested by the White settlers in South Africa
(c) Apartheid was strongly contested by Black Africans in South Africa
(d) Apartheid came to an end at the same time as Bantustans were created.
13. Ferguson notes that Transkei was like Lesotho in that
(a) Both were part of South Africas migrant labor system
(b) Both were Bantustans
(c) Both were created by an Apartheid-State
(d) Both were economically independent of South Africa.
14. Ferguson argues that in Transkei
(a) Poverty was depoliticized, unlike in Lesotho
(b) Poverty was politicized, unlike in Lesotho
(c) A real nation-state was constructed, unlike in Lesotho
(d) Apartheid was abolished, unlike in Lesotho.
15. Ferguson is critical about this claim made by anthropologists:
(a) All cultures are the same; hence it is hard to choose a field to study
(b) All cultures are unique; hence it is easy to compare cultures
(c) All cultures are unique; hence it is hard to compare different cultures
(d) All cultures are either the same or different; hence it is hard to compare cultures.
Please limit the answers to 200 words.
1. Wal-Mart is famous for requesting ever lower “price points” from its suppliers: If a supplier offers a product for $45.00, Wal-Mart will ask the supplier to consider introducing a similar product for $39.00. According to Heckscher-Ohlin Factor Theory, what consequences do such requests have?
2-he difference between domestic and international logistics
What are the elements that differentiate international logistics and domestic logistics?
3-What is the relationship between the growth in worldwide competition and the evolution of the supply chain concept?
For this assignment you will use ONLY AWK to create a program for summarizing and printing information
based on the directory listing data of files and information.
You are not to use any other programs, utilities, or scripting languages not covered in class, unless
otherwise specifically and explicitly stated in this document.
1. (30 points) A student working in Dr. Selbys research lab orders a new lab buffer system from Sigma chemical company. The chemical formula is proprietary and the manufacturer refuses to release the complete chemical formula. From the companys technical data sheet, the following information was provided, the buffer system is a triprotic buffer system with each proton being attached to a unique functional group that equilibrates as shown in the reaction below Sigma also includes that A represents the polyatomic chemical species that Sigma chemical refuses to release;
H3A + H2O H2A- + H3O+ HA-2 + H3O+ A-3 + H3O+
Its known that the A portion of the buffer has a molecular weight of 135.25 g/mol. The four components of the buffer system are packaged separately as dry powders with one in the fully protonated form and the others in the three sodium salt forms (H3A, NaH2A, Na2HA, and Na3A) where the sodium (atomic weight 22.99 g/mol) dissociates as a spectator ion when the buffer is prepared and does not participate in the acid-base equilibrium.
The student finds there are no pKa values on the packaging and gets upset. She cant Google the pKa values since she doesnt know the exact chemical formula, so she takes 182.23 grams of Na2HA and 64.58 grams of Na3A and measures the pH with her pH meter. She finds the pH is 8.5 after these components have mixed and equilibrated. This pH is too highugh!
She then takes 36.36 grams of Na2HA and 160.24 grams of NaH2A and finds the pH is 6.3. This pH is too lowugh!
She needs to prepare a buffer with a pH of 7.5 to show Dr. Selby her assay results in the morning. As she hurries around the lab due to this taking so long, she breaks the pH meter and cannot use it for the final buffer preparationugh!
She is left with only the values she has found from her previous measurements and her biochemistry class experience to guide her. She takes a deep breath, focuses, and asks herself;
How much, and which, dry compound(s) should I use to make 250 mL of my buffer at pH 7.5 that is 0.225 M?
Give her some assistance;
a. Show all relevant chemical equations involving the equilibrium expressions (5-points),
b. mathematical equations for your choice of which preliminary values to use (10-points), and
c. steps to find your final solution (15 points).
***Use the following page(s) for your answers. Add more pages as needed to the final document you submit.
2. (40 points) A student doing research at St. Petersburg College Cancer Center is working on a protein purification and kinetic assay for an enzyme found to be upregulated in patients with type-2 diabetes who have lung cancer. The protein is being prepared in a stabilized human cell line and the student has a significant amount of human cancer cells to prepare the enzyme.
When the enzyme is first isolated, the student uses gel filtration (GF, or size exclusion) chromatography and finds the molecular weight of the enzyme is 120,000 based on GF column standards. This form of the enzyme has 1,000 units of activity per mg of isolated protein. This result was repeated and verified.
The enzyme is purified a second time, but the student working with the GF column prior to the current purification has equilibrated the gel filtration column with a buffer containing a high concentration of urea. This was unknown at the time the enzyme was being purified, but was determined a few days later. This preparation showed a molecular weight of 40,000 based on GF column standards and had 2,000 units of activity per mg of protein. This result was repeated and verified.
The student was very confused and began considering changing her major. She then ran a SDS-PAGE gel with beta-mercaptoethanol added to both the sample that was found to be 120,000 MW on the GF column and the sample where urea was added where the molecular weight appeared to be 40,000 MW. These samples were run on the SDS-PAGE gel in separate lanes for analysis.
After staining her SDS-PAGE gel, she found the molecular weight of both her protein samples was 20,000 MW on the gel based on SDS-PAGE molecular weight standards. The student was even more confused and began perusing the course catalog for career options. She calls you to get career advice and explains her observations. You inform her you took biochemistry at St. Petersburg College and that the world needs devoted scientists right now more than ever–and she should not change her major. You tell her you can explain exactly whats occurring, and you do;
a. (10 points) How do you explain the different apparent molecular weights using the different methods based on your knowledge of protein structure? You must include both GF column purifications and the SDS-gel in your explanation.
b. (10 points) Is there a molecular difference in the enzyme activity for the 120,000 MW form and the 40,000 MW form of the enzyme? Which is more active and by how much?
c. (5 points) Is this an allosteric enzyme? What properties support it being allosteric from the data collected thus far? What experiments should be done to prove that it is? (2-3 sentences)
d. (5 points) Are there any likely post-translational modifications? What is the evidence for (or against) this?
e. (5 points) Show the structure(s) of the amino acids that might be modified based on the data collected (both prior and after modificationtwo total structures).
f. (5 points) What is the role of these post-translational modifications on the structure and/or function of the enzyme (one sentence)?
***Use the following page(s) for your answers. Add more pages as needed to the final document you submit.
3. (15-points) A student at St. Petersburg College isolates an enzyme with trypsin-like specificity from an organism that thrives in a volcanic deep sea vent where temperatures are typically above 100 Celsius. The student working with this enzyme finds there is no activity below 37 Celsius, only moderate activity around 50 Celsius, and optimal activity at 90 Celsius. He discusses these results with three other students who are attending the University of Florida, Florida State, and UCF and they exchange ideas on why this might be occurring. Student-A believes this is due to a completely different set of amino acids that exist in deep sea creatures which have special functional groups that only work at elevated temperatures. Student-B states the amino acids are the same as everywhere else on planet earth, but the temperature-activity observations are due to the pKa values that are only relevant at elevated temperatures and Student-C states this is due to the amount of Gibbs free energy that this organism needs for its chemical transitions due to its environment. As you listen to these ideas, you develop your own concepts related to the discussions in our biochemistry course, and decide which of these ideas are likely true are and which are not likely true;
a. Are Student-As ideas supported by any known biochemical information? Give 2-3 sentences why or why not these ideas are reasonable. (5 points)
b. Are Student-Bs ideas supported by any known biochemical information? Give 2-3 sentences why or why not these ideas are reasonable. (5 points)
c. Are Student-Cs ideas supported by any known biochemical information? Give 2-3 sentences why or why not these ideas are reasonable. (5 points)
***Use the remainder of this page and the following page for your answers
4. (25 points) The same enzyme from the question above is characterized using kinetic assays based on its specificity to develop a competitive inhibitor. Based on the specificity of trypsin substrates;
a. (5 points) Provide the complete chemical structure of an affinity-labeling molecule that might find use to determine which amino acids are near the active site.
b. (5- points) Provide the complete chemical structure of a chromogenic substrate molecule that might be used to determine the kinetics values of kcat and KM.
c. (5-points) Provide the complete chemical structure of a substrate analog that might find use as a reversible competitive inhibitor.
d. (5- points) Sketch the reaction scheme (E + S going to ES, and so on) for this inhibition and provide a double-reciprocal plot of the enzyme with substrate alone and with inhibitor added at three (3) increasing inhibitor concentrations.
e. (5- points) How do we determine if the inhibition is purely competitive? If the inhibitor works, but is not competitive, what might we observe on the graphs?
***Use the remainder of this page and the following page(s) for your answers. Add more pages as needed to the final document you submit.
